A DNA Nanostructure Platform for Directed Assembly of Synthetic Vaccines

被引:244
作者
Liu, Xiaowei [1 ,2 ,3 ]
Xu, Yang [3 ]
Yu, Tao [1 ,4 ]
Clifford, Craig [1 ,5 ]
Liu, Yan [2 ,3 ]
Yan, Hao [2 ,3 ]
Chang, Yung [1 ,5 ]
机构
[1] Arizona State Univ, Ctr Infect Dis & Vaccinol, Biodesign Inst, Tempe, AZ 85287 USA
[2] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA
[3] Arizona State Univ, Biodesign Inst, Ctr Single Mol Biophys, Tempe, AZ 85287 USA
[4] Sichuan Univ, W China Coll Stomatol, Dept Oral Maxillofacial Surg, Chengdu 610041, Sichuan Provinc, Peoples R China
[5] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA
关键词
DNA nanostructure; synthetic vaccine; mouse immunization; antibody response; immuno-adjuvant; DELIVERY; ORIGAMI;
D O I
10.1021/nl301877k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Safe and effective vaccines offer the best intervention for disease control. One strategy to maximize vaccine immunogenicity without compromising safety is to rationally design molecular complexes that mimic the natural structure of immunogenic microbes but without the disease-causing components. Here we use highly programmable DNA nanostructures as platforms to assemble a model antigen and CpG adjuvants together into nanoscale complexes with precise control of the valency and spatial arrangement of each element. Our results from immunized mice show that compared to a mixture of antigen and CpG molecules, the assembled antigen-adjuvant-DNA complexes induce strong and long-lasting antibody responses against the antigen without stimulating a reaction to the DNA nanostructure itself. This result demonstrates the potential of DNA nanostructures to serve as general platforms for the rational design and construction of a variety of vaccines.
引用
收藏
页码:4254 / 4259
页数:6
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