Identification of the glycosylation site of a major soybean allergen, Gly m Bd 30K

A major soybean allergen, Gly In Ed 30K, is a glycoprotein, A peptide containing a sugar chain was isolated from an alpha-chymotrypic hydrolyzate of the allergen. The amino acid sequence analysis of the peptide showed that its sugar chain binds to the Asn(170) residue. Furthermore, the sugar moiety of the allergen and peptide,vas shown to consist of mannose, N-acetylglucosamine, fucose, and xylose at a molar ratio of 3:2:1:1, These results indicate that Gly m Bd 30K is a N-linked glycoprotein.