Reprogramming somatic cells towards pluripotency by defined factors

被引:101
作者
Lewitzky, Marc [1 ]
Yamanaka, Shinya [1 ]
机构
[1] Kyoto Univ, Inst Frontier Med Sci, Sakyo Ku, Kyoto 6068507, Japan
基金
日本学术振兴会;
关键词
D O I
10.1016/j.copbio.2007.09.007
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The recent years have seen great advances in reversal of programming of differentiated somatic cells towards pluripotency by methods not involving nuclear transfer. Some of these may present a first step on the way to individual-based cell therapy without the problems connected to collection of mammalian unfertilised oocytes. Although differentiation of cells involves complex genetic and epigenetic changes, it is now possible to generate cells with many properties of pluripotent embryonic stem cells by retroviral transduction of differentiated cells with only four transcription factors: Oct3/4, Sox2, KIf4 and c-Myc. The re-programmed cells contribute to live chimeric mice and are transmitted via the germline.
引用
收藏
页码:467 / 473
页数:7
相关论文
共 59 条
[1]   Transcriptional regulation and transformation by MYC proteins [J].
Adhikary, S ;
Eilers, M .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2005, 6 (08) :635-645
[2]   Reprogramming somatic cells into stem cells [J].
Alberio, Ramiro ;
Campbell, Keith H. ;
Johnson, Andrew D. .
REPRODUCTION, 2006, 132 (05) :709-720
[3]   Multipotent cell lineages in early mouse development depend on SOX2 function [J].
Avilion, AA ;
Nicolis, SK ;
Pevny, LH ;
Perez, L ;
Vivian, N ;
Lovell-Badge, R .
GENES & DEVELOPMENT, 2003, 17 (01) :126-140
[4]   Synergy of SF1 and RAR in activation of Oct-3/4 promoter [J].
Barnea, E ;
Bergman, Y .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (09) :6608-6619
[5]  
BENSHUSHAN E, 1995, MOL CELL BIOL, V15, P1034
[6]   A bivalent chromatin structure marks key developmental genes in embryonic stem cells [J].
Bernstein, BE ;
Mikkelsen, TS ;
Xie, XH ;
Kamal, M ;
Huebert, DJ ;
Cuff, J ;
Fry, B ;
Meissner, A ;
Wernig, M ;
Plath, K ;
Jaenisch, R ;
Wagschal, A ;
Feil, R ;
Schreiber, SL ;
Lander, ES .
CELL, 2006, 125 (02) :315-326
[7]   Core transcriptional regulatory circuitry in human embryonic stem cells [J].
Boyer, LA ;
Lee, TI ;
Cole, MF ;
Johnstone, SE ;
Levine, SS ;
Zucker, JR ;
Guenther, MG ;
Kumar, RM ;
Murray, HL ;
Jenner, RG ;
Gifford, DK ;
Melton, DA ;
Jaenisch, R ;
Young, RA .
CELL, 2005, 122 (06) :947-956
[8]   Molecular control of pluripotency [J].
Boyer, Laurie A. ;
Mathur, Divya ;
Jaenisch, Rudolf .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 2006, 16 (05) :455-462
[9]   LIF/STAT3 controls ES cell self-renewal and pluripotency by a Myc-dependent mechanism [J].
Cartwright, P ;
McLean, C ;
Sheppard, A ;
Rivett, D ;
Jones, K ;
Dalton, S .
DEVELOPMENT, 2005, 132 (05) :885-896
[10]   Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells [J].
Chew, JL ;
Loh, YH ;
Zhang, WS ;
Chen, X ;
Tam, WL ;
Yeap, LS ;
Li, P ;
Ang, YS ;
Lim, B ;
Robson, P ;
Ng, HH .
MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (14) :6031-6046