Theory of peak capacity in gradient elution

被引：321

作者：

Neue, UD ^{[1
]}

机构：

[1] Waters Corp, Milford, MA 01757 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1079卷 / 1-2期

关键词：

peak capacity; gradient elution; theory of chromatography;

D O I：

10.1016/j.chroma.2005.03.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Peak capacity is the best measure of the performance of a gradient separation. In this paper, the theory of peak capacity for the standard operating conditions of reversed-phase and ion-exchange chromatography is outlined. The influence of the operating conditions on the peak capacity of a separation are discussed. Finally, bandspreading phenomena in gradient chromatography are analyzed. (c) 2005 Elsevier B.V. All rights reserved.

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页码：153 / 161

页数：9

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