Amperometric determination of xanthine and hypoxanthine at carbon electrodes. Effect of surface activity and the instrumental parameters on the sensitivity and the limit of detection

The performance of active graphite and carbon fiber surfaces produced by different mechanical/electrochemical methods of surface activation has been investigated in the amperometric determinations of xanthine and hypoxanthine under physiologically relevant conditions. The electrodes showed better limits of detection (LOD) when used with differential techniques with a capability of discriminating the analytical signal from the background. Square wave voltammetry and cyclic voltammetry showed the most sensitive response. Electrochemically activated carbon fiber ultramicroelectrodes showed the highest sensitivity (58 A M-1 cm(-2)) and the LOD in the 200 nM range was observed at the rough pyrolytic graphite electrodes by square wave voltammetry. The results demonstrate the feasibility of the development of new electroanalytical methods for the determination of oxypurines in biological samples. (C) 1999 Elsevier Science B.V. All rights reserved.