Antisense knockdown of PKC-α using LNA-oligos

被引：16

作者：

Hansen, JB ^{[1
]}

Westergaard, M ^{[1
]}

Thrue, CA ^{[1
]}

Giwercman, B ^{[1
]}

Oerum, H ^{[1
]}

机构：

[1] Cureon AS, DK-2100 Copenhagen 0, Denmark

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2003年 / 22卷 / 5-8期

关键词：

LNA; antisense; phosphorothioates; ISIS; 3521; protein kinase c-alpha; A549; cells;

D O I：

10.1081/NCN-120023045

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Full-length and 4 nucleotides truncated Locked Nucleic Acid (LNA) modifications of ISIS 3521 were compared for antisense properties in a cellular assay. ISIS 3521 is a 20-mer phosphorothioate designed to hybridise to human protein kinase G-alpha (PKC-alpha) mRNA and is currently submitted to clinical trials against cancer. We report that LNA can potentate this antisense oligo and retain the antisense potential with shorter oligos.

引用

页码：1607 / 1609

页数：3

共 4 条

[1]

DEAN NM, 1994, J BIOL CHEM, V269, P16416

[2] LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition [J].