Chiasma formation in Arabidopsis thaliana accession Wassileskija and in two meiotic mutants

被引:126
作者
Moran, ES
Armstrong, SJ
Santos, JL
Franklin, FCH
Jones, GH [1 ]
机构
[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England
[2] Univ Complutense Madrid, Dept Genet, E-28040 Madrid, Spain
关键词
Arabidopsis; chiasmata; FISH; meiosis; recombination;
D O I
10.1023/A:1009278902994
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Meiotic chiasmata were analysed in metaphase I pollen mother cells (PMCs) of wild-type Arabidopsis thaliana and in two meiotic mutants. Fluorescence in situ hybridisation (FISH) with 45S rDNA and 5S rDNA as probes was used to identify the five chromosome pairs. A wild-type chiasma frequency of 9.24 per cell was found, consistent with estimated genetic recombination values. Individual bivalent chiasma frequencies varied according to chromosome size; chromosome 1 had the highest mean chiasma frequency (2.14) while the short acrocentric chromosomes had the lowest frequencies (1.54 and 1.56). FISH analysis was extended to two meiotic mutants (asy1 and dsy1) having low residual bivalent and chiasma frequencies. Mutant dsy1 gave no indication of chromosome preference for residual bivalent formation; instead it showed a general reduction in bivalent and chiasma frequencies. In asy1, the longest chromosome (1) had the lowest bivalent frequency and chiasma frequency while the short acrocentric: chromosome 2 had the highest frequencies. This chromosome pair may be preferentially involved in synapsis and chiasma formation because of their association with the nucleolus. However, other factors may be operating since the other acrocentric chromosome (4), with similar size and structure to chromosome 2, did not share these chiasma properties.
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收藏
页码:121 / 128
页数:8
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