A protein phosphatase 2A inhibition assay for a fast and sensitive assessment of okadaic acid contamination in mussels

被引：123

作者：

Tubaro, A ^{[1
]}

Florio, C ^{[1
]}

Luxich, E ^{[1
]}

Sosa, S ^{[1
]}

DellaLoggia, R ^{[1
]}

Yasumoto, T ^{[1
]}

机构：

[1] TOHOKU UNIV, FAC AGR, AOBA KU, SENDAI, MIYAGI 981, JAPAN

来源：

TOXICON | 1996年 / 34卷 / 07期

关键词：

D O I：

10.1016/0041-0101(96)00027-X

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The specific inhibitory activity exerted by okadaic acid on protein phosphatase 2A was used to assess the presence of okadaic acid in mussels, using a commercially available protein phosphatase 2A preparation. Under the conditions used, okadaic acid inhibits the enzymatic activity dose-dependently, with an IC50 = 0.26 ng/ml (0.32 nM). The assay is accurate and reproducible. Okadaic acid was detected in concentrations as low as 0.063 ng/ml in aqueous solutions acid 2 ng/g in mussel digestive glands. Thirty naturally contaminated mussel samples were submitted to the protein phosphatase 2A inhibition assay as well as to an ELISA assay and to a MTT cytotoxicity assay, with similar results. The proposed assay is sensitive, rapid and does not require expensive equipment. These characteristics make it a good candidate for employment in the routine assessment of okadaic acid shellfish contamination. Copyright (C) 1996 Elsevier Science Ltd

引用

页码：743 / 752

页数：10

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