Identification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA

被引：25

作者：

Levy, H

Fisher, M

Ariel, N

Altboum, Z

Kobiler, D

机构：

[1] Israel Inst Biol Res, Dept Infect Dis, IL-70450 Ness Ziona, Israel

[2] Israel Inst Biol Res, Dept Biochem & Mol Genet, IL-70450 Ness Ziona, Israel

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 244卷 / 01期

关键词：

Bacillus anthracis; classification; RAPD; DNA; typing;

D O I：

10.1016/j.femsle.2005.01.039

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Classification and differentiation of Bacillus anthracis isolates by genetic markers play an important role in anthrax research. We used a PCR based method - Random Amplification of Polymorphic DNA (RAPD) - to identify genetic markers in B. anthracis strains. Twenty-five differential genetic markers were identified which divided the strains into five different groups. Three selected RAPD-markers were cloned and sequenced. The five RAPD-derived genotypes could be defined by integration of these three markers. This system offers a simple non-expensive method to classify B. anthracis strains in laboratories involved in the research of this bacterium. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：199 / 205

页数：7

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