Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays

被引：9

作者：

Yeghiazarian, T

Zhao, WQ

Read, SE

Kabat, W

Li, XY

Hamren, SJ

Sheridan, PJ

Wilber, JC

Chernoff, DN

Yogev, R

机构：

[1] Chiron Diagnost, Nucl Acid Diagnost & New Markers, Emeryville, CA 94608 USA

[2] Childrens Mem Hosp, Div Infect Dis, Sect Pediat & Maternal HIV1 Infect, Chicago, IL 60614 USA

[3] Northwestern Univ, Sch Med, Dept Microbiol & Immunol, Chicago, IL 60611 USA

[4] Northwestern Univ, Sch Med, Dept Pediat, Chicago, IL 60611 USA

[5] Univ Toronto, Hosp Sick Children, Div Infect Dis, Toronto, ON M5G 1X8, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 07期

关键词：

D O I：

10.1128/JCM.36.7.2096-2098.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have developed small-volume (50 or 250 mu l)-format branched-DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.

引用

页码：2096 / 2098

页数：3

共 7 条

[1]

[Anonymous], 1994, MMWR Recomm Rep, V43, P1

[2] An enhanced-sensitivity branched-DNA assay for quantification of human immunodeficiency virus type 1 RNA in plasma [J].

Kern, D ;

Collins, M ;

Fultz, T ;

Detmer, J ;

Hamren, S ;

Peterkin, JJ ;

Sheridan, P ;

Urdea, M ;

White, R ;

Yeghiazarian, T ;

Todd, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (12) :3196-3202

[3] Prognosis in HIV-1 infection predicted by the quantity of virus in plasma [J].

Mellors, JW ;

Rinaldo, CR ;

Gupta, P ;

White, RM ;

Todd, JA ;

Kingsley, LA .

SCIENCE, 1996, 272 (5265) :1167-1170

[4] RAPID AND PRECISE QUANTIFICATION OF HIV-1 RNA IN PLASMA USING A BRANCHED DNA SIGNAL AMPLIFICATION ASSAY [J].

PACHL, C ;

TODD, JA ;

KERN, DG ;

SHERIDAN, PJ ;

FONG, SJ ;

STEMPIEN, M ;

HOO, B ;

BESEMER, D ;

YEGHIAZARIAN, T ;

IRVINE, B ;

KOLBERG, J ;

KOKKA, R ;

NEUWALD, P ;

URDEA, MS .

JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY, 1995, 8 (05) :446-454

[5] HIV viral load markers in clinical practice [J].

Saag, MS ;

Holodniy, M ;

Kuritzkes, DR ;

OBrien, WA ;

Coombs, R ;

Poscher, ME ;

Jacobsen, DM ;

Shaw, GM ;

Richman, DD ;

Volberding, PA .

NATURE MEDICINE, 1996, 2 (06) :625-629

[6]

TODD J, 1995, J ACQ IMMUN DEF SYND, V10, pS35

[7]

TODD J, 1994, IMMUNOTHER INFECT DI, V6, P1

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