Structural characterization of a new lipid/DNA complex showing a selective transfection efficiency in ovarian cancer cells

被引：53

作者：

Caracciolo, G

Pozzi, D

Caminiti, R

Castellano, AC

机构：

[1] Univ Roma La Sapienza, Dipartimento Fis, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, INFM, I-00185 Rome, Italy

[3] Univ Roma Tor Vergata, Dipartimento Fis, I-00146 Rome, Italy

[4] Univ Roma Tor Vergata, INFM, I-00146 Rome, Italy

[5] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

来源：

EUROPEAN PHYSICAL JOURNAL E | 2003年 / 10卷 / 04期

关键词：

D O I：

10.1140/epje/i2002-10117-x

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We investigated, for the first time, by using Energy Dispersive X-ray Diffraction, the structure of a new ternary cationic liposome formulated with dioleoyl trimethylammonium propane (DOTAP), 1,2-dioleoyl-3-phosphatidylethanolamine (DOPE) and cholesterol (Chol) (DDC) which has been recently found to have a selective high gene transfer ability in ovarian cancer cells. Our structural results provide a further experimental support to the widely accepted statement that there is not a simple and direct correlation between structure and transfection efficiency and that the factors controlling cationic lipid/DNA (CL-DNA) complexes-mediated gene transfer depend not only on the formulations of the cationic liposomes and their thermodynamic phase, but also significantly on the cell properties.

引用

页码：331 / 336

页数：6

共 34 条

[1] 3β [N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol)-mediated gene delivery to primary rat neurons:: Characterization and mechanism [J].