In vivo assessment of microvascular nitric oxide production and its relation with blood flow

被引:20
作者
Figueroa, XF [1 ]
Martínez, AD [1 ]
González, DR [1 ]
Jara, PI [1 ]
Ayala, S [1 ]
Boric, MP [1 ]
机构
[1] Pontificia Univ Catolica Chile, Dept Ciencias Fisiol, Fac Ciencias Biol, Unidad Reulac Neurohumoral, Santiago 6513492, Chile
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2001年 / 280卷 / 03期
关键词
nitric oxide chemiluminescence; subcellular endothelial nitric oxide synthase distribution; N-omega-nitro-L-arginine; vascular conductance; hamster cheek pouch;
D O I
10.1152/ajpheart.2001.280.3.H1222
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To assess the hypothesis that microvascular nitric oxide (NO) is critical to maintain blood flow and solute exchange, we quantified NO production in the hamster cheek pouch in vivo, correlating it with vascular dynamics. Hamsters (100-120 g) were anesthetized and prepared for measurement of microvessel diameters by intravital microscopy, of plasma flow by isotopic sodium clearance, and of NO production by chemiluminescence. Analysis of endothelial NO synthase (eNOS) location by immunocytochemistry and subcellular fractionation revealed that eNOS was present in arterioles and venules and was 67 +/- 7% membrane bound. Basal NO release was 60.1 +/- 5.1 pM/min (n = 35), and plasma flow was 2.95 +/- 0.27 mul/min (n = 29). Local NO synthase inhibition with 30 muM N-omega-nitro-L-arginine reduced NO production to 8.6 +/- 2.6 pmol/min (-83 +/- 5%, n = 9) and plasma flow to 1.95 +/- 0.15 mul/min (-28 +/- 12%, n = 17) within 30-45 min, in parallel with constriction of arterioles (9-14%) and venules (19-25%). The effects of N-omega-nitro-L-arginine (10-30 muM) were proportional to basal microvascular conductance (r = 0.7, P< 0.05) and fully prevented by 1 mM L-arginine. We conclude that in this tissue, NO production contributes to 35-50% of resting microvascular conductance and plasma-tissue exchange.
引用
收藏
页码:H1222 / H1231
页数:10
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