New device and method for capture, reverse transcription and nested PCR in a single closed-tube

被引：76

作者：

Olmos, A ^{[1
]}

Cambra, M ^{[1
]}

Esteban, O ^{[1
]}

Gorris, MT ^{[1
]}

Terrada, E ^{[1
]}

机构：

[1] Inst Valenciano Invest Agr, Dept Protecc Vegetal & Biotecnol, E-46113 Moncada, Valencia, Spain

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 06期

关键词：

D O I：

10.1093/nar/27.6.1564

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A device and improved method based on the use of a compartmentalized Eppendorf tube that allows capture, reverse transcription and nested-PCR in a single closed-tube has been developed and patented. The main advantages of the system are the high sensitivity obtained, the simplicity, the low risk of contamination and the easy establishment of adequate conditions for nested-PCR. The method has been successfully applied to the detection and characterization of citrus tristeza closterovirus and plum pox potyvirus isolates in plant tissues and single aphids squashed on paper. This device and methodology could be easily adapted to the detection of other targets.

引用

页码：1564 / 1565

页数：2

共 12 条

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