Janibacter limosus gen nov, sp nov, a new actinomycete with meso-diaminopimelic acid in the cell wall

New gram-positive bacteria were isolated from 1-year-old sludge from a wastewater treatment plant, The isolates are coccoid to rod-shaped, nonmotile aerobes that form neither spores nor mycelia. They are characterized by a peptidoglycan with directly cross-linked meso-diaminopimelic acid (type A1 gamma), by the presence of menaquinone MK-8(H-4), and by the lack of mycolic acids, The strains have complex fatty acid patterns with i-C-16:0 and straight-chain saturated and unsaturated fatty acids as major components, The G+C content of the DNA is 70 mol%. The results of chemotaxonomic studies and a 16S ribosomal DNA sequence comparison support our proposal to assign these bacteria to a new genus, the genus Janibacter gen, nov.; the type species is Janibacter limosus sp. nov., and the type strain of J. limosus is strain HKI 83 (= DSM 11140).