Patterns of gene expressions induced by arsenic trioxide in cultured human fibroblasts

被引：20

作者：

Burnichon, V

Jean, S

Bellon, L

Maraninchi, M

Bideau, C

Orsière, T

Margotat, A

Gérolami, V

Botta, A

Bergé-Lefranc, JL

机构：

[1] Univ Mediterranee, Fac Med, Lab Biogenotoxicol & Mutagenese Environm, EA 1784,IFR PMSE 112, F-13385 Marseille 05, France

[2] Hop Conception, Lab Biochim & Biol Mol, F-13385 Marseille 05, France

[3] Fac Med Marseille, INSERM, U476, IFR 35, F-13385 Marseille 05, France

来源：

TOXICOLOGY LETTERS | 2003年 / 143卷 / 02期

关键词：

arsenic trioxide; real time RT-PCR; early transient expression; delayed gene expression;

D O I：

10.1016/S0378-4274(03)00171-1

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Arsenic exposure is associated with several human diseases and particularly, with neoplasia. Although the mechanism of arsenic toxicity is not fully understood, several recent works pointed out the involvement of oxidative stress in arsenic-induced DNA damage that, in living cells, correlates with changes in gene expressions. In cultured human fibroblasts exposed for 24 It to micromolar arsenic concentrations, we studied, using real-time RT-PCR, the expression profile of a limited number of genes: genes coding for a stress protein (HSP70), transcription factors (cJUN, cFOS, ETR103, ETR101 and TTP) and cell cycle or DNA repair proteins (P21, GADD153). We observed that the expression profile of genes followed individual different patterns that can be summed up in early-transient gene expression by contrast to delayed gene expression. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：155 / 162

页数：8

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