Manipulating ribulose bisphosphate carboxylase/oxygenase in the chloroplasts of higher plants

被引：93

作者：

Andrews, TJ ^{[1
]}

Whitney, SM ^{[1
]}

机构：

[1] Australian Natl Univ, Res Sch Biol Sci, Canberra, ACT 2601, Australia

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2003年 / 414卷 / 02期

关键词：

Rubisco; plastome; chloroplast; tobacco; transgenic plants; transplastomic plants; photosynthesis; antisense RNA;

D O I：

10.1016/S0003-9861(03)00100-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transgenic manipulation of the photosynthetic CO2-fixing enzyme, ribulose bisphosphate carboxylase/oxygenase (Rubisco) in higher plants provides a very specific means of testing theories about photosynthesis and its regulation. It also encourages prospects for radically improving the efficiencies with which photosynthesis and plants use the basic resources of light, water, and nutrients. Manipulation was once limited to variation of the leaf's total content of Rubisco by transforming the nucleus with antisense genes directed at the small subunit. More recently, technology for transforming the small genome of the plastid of tobacco has enabled much more precise manipulation and replacement of the plastome-encoded large subunit. Engineered changes in Rubisco's properties in vivo are reflected as profound changes in the photosynthetic gas-exchange properties of the leaves and the growth requirements of the plants. Unpredictable expression of plastid transgenes and assembly requirements of some foreign Rubiscos that are not satisfied in higher-plant plastids provide challenges for future research. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：159 / 169

页数：11

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