ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool

被引：254

作者：

Sander, Jeffry D. ^{[1
,2
,3
]}

Maeder, Morgan L. ^{[1
,2
,4
]}

Reyon, Deepak ^{[5
]}

Voytas, Daniel F. ^{[6
]}

Joung, J. Keith ^{[1
,2
,3
,4
]}

Dobbs, Drena ^{[5
]}

机构：

[1] Massachusetts Gen Hosp, Mol Pathol Unit, Ctr Canc Res, Charlestown, MA 02129 USA

[2] Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Charlestown, MA 02129 USA

[3] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

[4] Harvard Univ, Sch Med, Biol & Biomed Sci Program, Boston, MA 02115 USA

[5] Iowa State Univ, Dept Genet Dev & Cell Biol, Interdept Grad Program Bioinformat & Computat Bio, Ames, IA 50011 USA

[6] Univ Minnesota, Dept Genet Cell Biol & Dev, Ctr Genome Engn, Minneapolis, MN 55455 USA

来源：

NUCLEIC ACIDS RESEARCH | 2010年 / 38卷

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

ARTIFICIAL TRANSCRIPTION FACTORS; DNA-RECOGNITION; ZIF268-DNA COMPLEXES; RESTRICTION ENZYMES; CRYSTAL-STRUCTURE; NUCLEASES; CONSTRUCTION; PROTEINS; CLEAVAGE; DOMAINS;

D O I：

10.1093/nar/gkq319

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ZiFiT (Zinc Finger Targeter) is a simple and intuitive web-based tool that provides an interface to identify potential binding sites for engineered zinc finger proteins (ZFPs) in user-supplied DNA sequences. In this updated version, ZiFiT identifies potential sites for ZFPs made by both the modular assembly and OPEN engineering methods. In addition, ZiFiT now integrates additional tools and resources including scoring schemes for modular assembly, an interface with the Zinc Finger Database (ZiFDB) of engineered ZFPs, and direct querying of NCBI BLAST servers for identifying potential off-target sites within a host genome. Taken together, these features facilitate design of ZFPs using reagents made available to the academic research community by the Zinc Finger Consortium. ZiFiT is freely available on the web without registration at http://bindr.gdcb.iastate.edu/ZiFiT/.

引用

页码：W462 / W468

页数：7

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