Gram-chromotrope: A new technique that enhances detection of microsporidial spores in clinical samples

被引:44
作者
Moura, H
DaSilva, JLN
Sodre, FC
Brasil, P
Wallmo, K
Wahlquist, S
Wallace, S
Croppo, GP
Visvesvara, GS
机构
[1] UNIV ESTADO RIO DE JANEIRO, FAC CIENCIAS MED, DEPT PATOL & LAB, RIO DE JANEIRO, BRAZIL
[2] FIOCRUZ MS, INST OSWALDO CRUZ, HOSP EVANDRO CHAGAS, BR-21045900 RIO DE JANEIRO, BRAZIL
[3] CTR DIS CONTROL, DIV PARASIT DIS, NATL CTR INFECT DIS, ATLANTA, GA 30333 USA
关键词
D O I
10.1111/j.1550-7408.1996.tb05019.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have developed a new staining procedure that combines the traditional Gram staining for bacteria and the Weber's chromotrope staining method, the standard technique for the detection of microsporidia spores in clinical specimens. This ''Gram-chromotrope'' staining technique the staining characteristics of microsporidia spores and facilitates the easy detection and differentiation of spores from other microorganisms that are found in clinical specimens, especially stool samples. This new technique is fast, reliable, and simple to perform, and can be easily adapted for use in clinical laboratories.
引用
收藏
页码:S94 / S95
页数:2
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