The detection of antibodies against Schistosoma mansoni soluble egg antigens (SEA) and CEF6 in ELISA, before and after chemotherapy

被引：18

作者：

Doenhoff, MJ

Wheeler, JG

Tricker, K

Hamilton, JV

Sturrock, RF

Butterworth, AE

Ouma, JH

Mbugua, GG

Kariuki, C

Koech, D

机构：

[1] Univ Wales Bangor, Sch Biol Sci, Bangor LL57 2UW, Gwynedd, Wales

[2] Univ Forvie Site, Dept Publ Hlth & Primary Care, Cambridge CB2 2SR, England

[3] Manchester Royal Infirm, Dept Psychiat & Behav Sci, HERaM, Manchester M13 9WL, Lancs, England

[4] Univ London London Sch Hyg & Trop Med, London WC1E 7HT, England

[5] Biomed Res & Training Inst, Harare, Zimbabwe

[6] Kenya Minist Hlth, Div Vector Borne Dis, Nairobi, Kenya

[7] Kenya Govt Med Res Ctr, Nairobi, Kenya

来源：

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY | 2003年 / 97卷 / 07期

关键词：

D O I：

10.1179/000349803225002354

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Circulating IgG antibodv reactivity and excreted egg counts were investigated in 489 Kenyans given chemotherapy for schistosomiasis mansoni. Antibody reactivity was measured in ELISA, using either unfractionated aqueous-soluble constituents of Schistosoma mansoni eggs (SEA) or CEF6 (a soluble fraction of S. mansoni eggs containing two cationic antigens) as the antigen source. Antibody reactivity for each antigen source was strongly associated with egg counts, both pre- and post-treatment. Approximately 6 months after chemotherapy, egg counts were zero in 84% of the subjects. The mean optical densities (OD) measured in the post-treatment ELISA were 60% (CEF6) or 45% (SEA) lower than the pre-treatment values, the reduction in the OD with CEF6 as antigen source being significantly greater than that observed with SEA (P < 0.001). The usefulness of an assay for antibody reactivity in monitoring the effects of the treatment of schistosomiasis is discussed.

引用

页码：697 / 709

页数：13

共 31 条

[1] COMPARISON OF DIFFERENT CHEMOTHERAPY STRATEGIES AGAINST SCHISTOSOMA-MANSONI IN MACHAKOS DISTRICT, KENYA - EFFECTS ON HUMAN INFECTION AND MORBIDITY [J].