Modified Multiple-Locus Variable-Number Tandem-Repeat Analysis for Rapid Identification and Typing of Clostridium difficile during Institutional Outbreaks

被引：10

作者：

Broukhanski, George

Low, Donald E.

Pillai, Dylan R.

机构：

[1] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[2] Ontario Agcy Hlth Protect & Promot, Toronto, ON, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 05期

关键词：

FIELD GEL-ELECTROPHORESIS; PCR; EPIDEMIC; STRAINS; TOXIN; INFECTION; DIARRHEA; CANADA; GENES;

D O I：

10.1128/JCM.02359-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A modified multiple-locus variable-number tandem-repeat analysis (MMLVA) method was validated on Clostridium difficile-infected stool specimens from institutional outbreaks. The method allows simultaneous detection of toxin genes, deletions, and tandem repeats from cultured isolates or stool specimens. Results were used to aid institutional outbreak investigation by identifying clusters of NAP1/027.

引用

页码：1983 / 1986

页数：4

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