A new mitochondrial fluorescent zinc sensor

被引：122

作者：

Sensi, SL

Ton-That, D

Weiss, JH

Rothe, A

Gee, KR

机构：

[1] Mol Probes Inc, Eugene, OR 97402 USA

[2] Univ Calif Irvine, Dept Neurol, Irvine, CA 92697 USA

[3] Univ G dAnnunzio, Dept Neurol, CESI Ctr Excellence Aging, I-66013 Chieti, Italy

来源：

CELL CALCIUM | 2003年 / 34卷 / 03期

关键词：

fluorescence; intracellular; neuron; Zn2+; RhodZin-3;

D O I：

10.1016/S0143-4160(03)00122-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A novel cationic fluorescent zinc (Zn2+) indicator (RhodZin-3) with nanomolar affinity for Zn2+ has been synthesized. RhodZin-3 exhibits large pH-independent fluorescence increases in the orange region of the visible wavelength spectrum with increasing zinc concentrations, and no sensitivity to physiolovically relevant Ca2+ concentrations. Experiments in neuronal cell cultures show that RhodZin-3 effectively localizes into mitochondria and detects changes of intramitochondrial free Zn2+ ([Zn2+](m)). (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：281 / 284

页数：4

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