Studies of clusters and biomolecules in ELISA

被引:14
作者
Andersen, JU [1 ]
Andersen, LH
Hvelplund, P
Lapierre, A
Moller, SP
Nielsen, SB
Pedersen, UV
Tomita, S
机构
[1] Univ Aarhus, Dept Phys & Astron, DK-8000 Aarhus C, Denmark
[2] Univ Aarhus, Inst Storage Ring Facilities ISA, DK-8000 Aarhus, Denmark
来源
HYPERFINE INTERACTIONS | 2003年 / 146卷 / 1-4期
关键词
storage ring; cluster; biomolecule; green fluorescent protein; nucleotide; dissociation; spectroscopy; lifetime;
D O I
10.1023/B:HYPE.0000004201.81619.ab
中图分类号
O64 [物理化学(理论化学)、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号
070203 ; 070304 ; 081704 ; 1406 ;
摘要
Application of the electrostatic ion storage ring ELISA to studies of clusters and biomolecules is discussed. Ions injected from a plasma source or a sputter source are hot, and at short times the yield of neutrals is usually dominated by decay of metastable ions. We have demonstrated that the decay function is close to a 1/t dependence when the internal energy of the ions is conserved, i.e., when photon emission can be ignored. Deviations from a 1/t distribution therefore gives information about the radiative lifetime or, for larger systems, about the intensity of the emitted radiation. Systematic measurements have been carried out for fullerene anions C(N)(-), for even values of N from 36 to 96, to test a classical dielectric model. Recently we have installed an electrospray ion source with a Paul trap for bunching, which can be used to inject biomolecular ions from solution, and the first experiments on laser spectroscopy of biomolecules have been carried out. Also lifetimes of excited states have been measured for stored biomolecular ions excited by a laser pulse.
引用
收藏
页码:283 / 291
页数:9
相关论文
共 31 条
[1]  
ALMEN O, 1957, NUCL INSTRUM METHODS, V1, P302
[2]   PIG SPUTTER SOURCE FOR NEGATIVE-IONS [J].
ANDERSEN, HH ;
TYKESSON, P .
IEEE TRANSACTIONS ON NUCLEAR SCIENCE, 1975, NS22 (03) :1632-1636
[3]   Radiative cooling of C-60 [J].
Andersen, JU ;
Brink, C ;
Hvelplund, P ;
Larsson, MO ;
Nielsen, BB ;
Shen, H .
PHYSICAL REVIEW LETTERS, 1996, 77 (19) :3991-3994
[4]   The combination of an electrospray ion source and an electrostatic storage ring for lifetime and spectroscopy experiments on biomolecules [J].
Andersen, Jens Ulrik ;
Hvelplund, Preben ;
Nielsen, Steen Brondsted ;
Tomita, Shigeo ;
Wahlgreen, Helge ;
Moller, Soren Pape ;
Pedersen, Ulrik Vindelev ;
Forster, James S. ;
Jorgensen, Thomas J.D. .
Review of Scientific Instruments, 2002, 73 (3 I)
[5]   Radiative cooling of fullerene anions in a storage ring [J].
Andersen, JU ;
Gottrup, C ;
Hansen, K ;
Hvelplund, P ;
Larsson, MO .
EUROPEAN PHYSICAL JOURNAL D, 2001, 17 (02) :189-204
[6]   Classical dielectric models of fullerenes and estimation of heat radiation [J].
Andersen, JU ;
Bonderup, E .
EUROPEAN PHYSICAL JOURNAL D, 2000, 11 (03) :413-434
[7]  
Andersen LH, 1997, ADV ATOM MOL OPT PHY, V38, P155
[8]   Fragmentation of isolated ions by multiple photon absorption: A quantitative study [J].
Boissel, P ;
deParseval, P ;
Marty, P ;
Lefevre, G .
JOURNAL OF CHEMICAL PHYSICS, 1997, 106 (12) :4973-4984
[9]   INFRARED RADIATIVE COOLING OF GAS-PHASE IONS [J].
DUNBAR, RC .
MASS SPECTROMETRY REVIEWS, 1992, 11 (04) :309-339
[10]  
Gaskell SJ, 1997, J MASS SPECTROM, V32, P677, DOI 10.1002/(SICI)1096-9888(199707)32:7<677::AID-JMS536>3.3.CO