Microorganism inactivation using high-pressure generation in sealed vessels under sub-zero temperature

被引:39
作者
Hayakawa, K
Ueno, Y
Kawamura, S
Kato, T
Hayashi, R [1 ]
机构
[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Lab Biomacromol Chem,Sakyo Ku, Kyoto 60601, Japan
[2] Kyoto Prefectural Comprehens Guidance Ctr Small &, Shimogyo Ku, Kyoto 600, Japan
[3] San Contact Lens Ltd, Nakagyo Ku, Kyoto 604, Japan
关键词
High Pressure; Lactobacillus; Aspergillus; Staphylococcus Aureus; Saccharomyces Cerevisiae;
D O I
10.1007/s002530051313
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In order to test the possibility of utilizing high pressure in bioscience and biotechnology, a simple method for high-pressure generation and its use for microbial inactivation have been studied. When a pressure vessel was filled with water, sealed tightly and cooled to sub-zero temperatures, high pressure was generated in the vessel. The pressure generation was 60 MPa at -5 degrees C, 103 MPa at -10 degrees C, and 140 MPa at -15 degrees C, -20 degrees C, and -22 degrees C. The high pressure generated inactivated microorganisms effectively: yeasts (Saccharomyces cerevisiae and Zygosaccharomyces rouxii), bacteria (Lactobacillus brevis and Eschericia coli), and fungi (Aspergillus niger and Aspergillus oryzae) were completely inactivated when stored in sealed vessels -20 degrees C for 24 h. However, Staphylococcus aureus was only partly inactivated under the same conditions. This method opens up a new application of high pressure for storing, transporting, and sterilizing of foods and biological materials.
引用
收藏
页码:415 / 418
页数:4
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