Varicella-zoster virus DNA in cells isolated from human trigeminal ganglia

被引：53

作者：

Levin, MJ

Cai, GY

Manchak, MD

Pizer, LI

机构：

[1] Univ Colorado, Hlth Sci Ctr, Sch Med, Sect Pediat Infect Dis, Denver, CO 80262 USA

[2] Univ Colorado, Dept Microbiol, Sch Med, Denver, CO 80262 USA

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 12期

关键词：

D O I：

10.1128/JVI.77.12.6979-6987.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To determine the type of cell(s) that contain latent varicella-zoster virus (VZV) DNA, we prepared pure populations of neurons and satellite cells from trigeminal ganglia of 18 humans who had previously had a VZV infection. VZV DNA was present in 34 of 2,226 neurons (1.5%) and in none of 20,700 satellite cells. There was an average of 4.7 (range of 2 to 9) copies of VZV DNA per latently infected neuron. Latent VZV DNA was primarily present in large neurons, whereas the size distribution of herpes simplex virus DNA was markedly different.

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页码：6979 / 6987

页数：9

相关论文

共 36 条

[1] Fractionation of neurons and satellite cells from human sensory ganglia in order to study herpesvirus latency [J].

Cai, GY ;

Pizer, LI ;

Levin, MJ .

JOURNAL OF VIROLOGICAL METHODS, 2002, 104 (01) :21-32

[2] Varicella-zoster virus (VZV) transcription during latency in human ganglia: Detection of transcripts mapping to genes 21, 29, 62, and 63 in a cDNA library enriched for VZV RNA [J].

Cohrs, RJ ;

Barbour, M ;

Gilden, DH .

JOURNAL OF VIROLOGY, 1996, 70 (05) :2789-2796

[3] VARICELLA-ZOSTER VIRUS (VZV) TRANSCRIPTION DURING LATENCY IN HUMAN GANGLIA - PREVALENCE OF VZV GENE-21 TRANSCRIPTS IN LATENTLY INFECTED HUMAN GANGLIA [J].

COHRS, RJ ;

BARBOUR, MB ;

MAHALINGAM, R ;

WELLISH, M ;

GILDEN, DH .

JOURNAL OF VIROLOGY, 1995, 69 (04) :2674-2678

[4] Analysis of individual human trigeminal ganglia for latent herpes simplex virus type 1 and varicella-zoster virus nucleic acids using real-time PCR [J].

Cohrs, RJ ;

Randall, J ;

Smith, J ;

Gilden, DH ;

Dabrowski, C ;

van der Keyl, H ;

Tal-Singer, R .

JOURNAL OF VIROLOGY, 2000, 74 (24) :11464-11471

[5] PATTERNS OF GENE-EXPRESSION AND SITES OF LATENCY IN HUMAN NERVE GANGLIA ARE DIFFERENT FOR VARICELLA-ZOSTER AND HERPES-SIMPLEX VIRUSES [J].

CROEN, KD ;

OSTROVE, JM ;

DRAGOVIC, LJ ;

STRAUS, SE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) :9773-9777

[6] Quantitation of varicella-zoster virus DNA in whole blood, plasma, and serum by PCR and electrochemiluminescence [J].

de Jong, MD ;

Weel, JFL ;

Schuurman, T ;

Wertheim-van Dillen, PM ;

Boom, R .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (07) :2568-2573

[7] DETECTION OF HERPES-SIMPLEX VIRUS-SPECIFIC DNA-SEQUENCES IN LATENTLY INFECTED MICE AND IN HUMANS [J].

EFSTATHIOU, S ;

MINSON, AC ;

FIELD, HJ ;

ANDERSON, JR ;

WILDY, P .

JOURNAL OF VIROLOGY, 1986, 57 (02) :446-455

[8]

FORSTER E, 1994, BIOTECHNIQUES, V16, P18

[9] DETECTION OF VARICELLA-ZOSTER VIRUS-DNA IN HUMAN GENICULATE GANGLIA BY POLYMERASE CHAIN-REACTION [J].

FURUTA, Y ;

TAKASU, T ;

FUKUDA, S ;

SATOMATSUMURA, KC ;

INUYAMA, Y ;

HONDO, R ;

NAGASHIMA, K .

JOURNAL OF INFECTIOUS DISEASES, 1992, 166 (05) :1157-1159

[10]

Furuta Y, 1997, J MED VIROL, V51, P214, DOI 10.1002/(SICI)1096-9071(199703)51:3&lt

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