Association between intraepithelial Escherichia coli and colorectal cancer

被引:259
作者
Swidsinski, A
Khilkin, M
Kerjaschki, D
Schreiber, S
Ortner, M
Weber, J
Lochs, H
机构
[1] Humboldt Univ, Med Klin 4, Charite, Schwerpunkt Gastroenterol, D-10098 Berlin, Germany
[2] Univ Vienna, Inst Klin Pathol, Vienna, Austria
关键词
D O I
10.1016/S0016-5085(98)70194-5
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background & Aims: Although multiple studies have focused on Helicobacter pylori, little is known about the mucosa-associated flora of the colon. The aim of this study was to detect bacteria directly in colonic mucosa from patients screened for colorectal cancer. Methods: Bacteria were quantified with the polymerase chain reaction and identified by comparative sequence analysis in colonoscopic biopsy specimens from 31 asymptomatic and 34 symptomatic controls with normal colonoscopic findings, 29 patients with colonic adenoma, and 31 patients with colorectal carcinoma. In 41 patients, intra- and extracellular location of bacteria was confirmed with the gentamicin protection assay. Results: No bacteria were detected in biopsy specimens from 97% of asymptomatic and 69% of symptomatic controls. In contrast, bacterial concentrations of 10(3)-10(5) colony-forming units per microliter were detected in biopsy specimens from both malignant and macroscopically normal tissue in 90% and 93% of patients with adenoma and carcinoma, respectively. E. coli and coli-like bacteria were shown to colonize the colonic mucosa in 82% of these patients. The gentamicin protection assay indicated that E. coli was partially intracellular in 87% of patients with adenoma and carcinoma and in none of the controls. Conclusions: The colonic mucosa of patients with colorectal carcinoma but not normal colonic mucosa is colonized by intracellular E. coli.
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页码:281 / 286
页数:6
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