Allogeneic virus-specific T cells with HLA alloreactivity do not produce GVHD in human subjects

被引：156

作者：

Melenhorst, J. Joseph ^{[2
]}

Leen, Ann M. ^{[1
,3
]}

Bollard, Catherine M. ^{[1
,3
]}

Quigley, Maire F. ^{[4
,5
]}

Price, David A. ^{[4
,5
]}

Rooney, Cliona M. ^{[1
,3
]}

Brenner, Malcolm K. ^{[1
,3
]}

Barrett, A. John ^{[2
]}

Heslop, Helen E. ^{[1
,3
]}

机构：

[1] Texas Childrens Hosp, Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA

[2] NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA

[3] Methodist Hosp, Houston, TX 77030 USA

[4] Cardiff Univ, Sch Med, Cardiff, S Glam, Wales

[5] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA

来源：

BLOOD | 2010年 / 116卷 / 22期

基金：

美国国家卫生研究院;

关键词：

VERSUS-HOST-DISEASE; LYMPHOPROLIFERATIVE DISEASE; TRANSPLANTATION; CYTOMEGALOVIRUS; LYMPHOCYTES; RECIPIENTS; THERAPY; PREVENT; DONOR;

D O I：

10.1182/blood-2010-06-289991

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Adoptive transfer of viral antigen-specific memory T cells can reconstitute antiviral immunity, but in a recent report a majority of virus-specific cytotoxic T-lymphocyte (CTL) linesshowedin vitro cross-reactivity against allo-human leukocyte antigen (HLA) molecules as measured by interferon-gamma secretion. We therefore reviewed our clinical experience with adoptive transfer of allogeneic hematopoietic stem cell transplantation donor-derived virus-specific CTLs in 153 recipients, including 73 instances where there was an HLA mismatch. There was no de novo acute graft-versus-host disease after infusion, and incidence of graft-versus-host disease reactivation was low and not significantly different in recipients of matched or mismatched CTL. However, we found that virus-specific T cell lines recognized up to 10% of a panel of 44 HLA disparate targets, indicating that virus-specific T cells can have cross-reactivity with HLA-mismatched targets in vitro. These data indicate that the adoptive transfer of partially HLA-mismatched virus-specific CTL is safe despite in vitro recognition of recipient HLA molecules. (Blood. 2010; 116(22): 4700-4702)

引用

页码：4700 / 4702

页数：3

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