Red blood cells promote survival and cell cycle progression of human peripheral blood T cells independently of CD58/LFA-3 and heme compounds

被引:41
作者
Fonseca, AM
Pereira, CF
Porto, G
Arosa, FA
机构
[1] Inst Mol & Cell Biol, IBMC, Lab Mol Immunol, Oporto, Portugal
[2] ICBAS, P-4150180 Oporto, Portugal
[3] Santo Antonio Gen Hosp, Oporto, Portugal
关键词
red blood cell; T cell; CD8+; survival; cell cycle; apoptosis; CD58/LFA-3; heme; REACTIVE ANTIBODY-REPERTOIRES; INTESTINAL EPITHELIAL-CELLS; HUMAN LYMPHOCYTES-T; AUTOLOGOUS ERYTHROCYTES; CYTOKINE SECRETION; STIMULATORY PROPERTIES; ROSETTE FORMATION; INDUCED APOPTOSIS; LFA-3; MOLECULES; IRON-DEFICIENCY;
D O I
10.1016/S0008-8749(03)00170-9
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Red blood cells (RBC) are known to modulate T cell proliferation and function possibly through downregulation of oxidative stress. By examining parameters of activation, division, and cell death in vitro, we show evidence that the increase in survival afforded by RBC is due to the maintenance of the proliferative capacity of the activated T cells. We also show that the CD3+CD8+ T cell subset was preferentially expanded and rescued from apoptosis both in bulk peripheral blood lymphocyte cultures and with highly purified CD8+ T cells. The ability of RBC to induce survival of dividing T cells was not affected by blocking the CD58/CD2 interaction. Moreover, addition of hemoglobin, heme or protoporphyrin IX to cultures of activated T cells did not reproduce the effect of intact RBC. Considering that RBC circulate throughout the body, they could play a biological role in the modulation of T cell differentiation and survival in places of active cell division. Neither CD58 nor the heme compounds studied seem to play a direct relevant role in the modulation of T cell survival. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:17 / 28
页数:12
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