Comparison of fatty acid methyl ester analysis with the use of API 20E and NFT strips for identification of aquatic bacteria

被引：23

作者：

Brown, BJ ^{[1
]}

Leff, LG ^{[1
]}

机构：

[1] KENT STATE UNIV,WATER RESOURCES RES INST,KENT,OH 44242

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 06期

关键词：

D O I：

10.1128/AEM.62.6.2183-2185.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aquatic bacteria grown on MacConkey agar and modified nutrient agar were identified by using API 20E and NFT strips and fatty acid methyl ester (FAME) analysis. Identifications agreed at the species level 35.7% of the time when API 20E strips and FAME analysis were used and in 4.3% of the cases when API NFT strips and FAME analysis were used. These techniques require further development before extended use in ecological studies.

引用

页码：2183 / 2185

页数：3

共 16 条

[1]

Amann R., 1994, ASM News (Washington), V60, P360

[2] PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].

AMANN, RI ;

LUDWIG, W ;

SCHLEIFER, KH .

MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169

[3] EVALUATION OF THE RAPID NFT SYSTEM FOR IDENTIFICATION OF GRAM-NEGATIVE, NONFERMENTING RODS [J].

APPELBAUM, PC ;

LEATHERS, DJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1984, 20 (04) :730-734

[4]

BERGAN T, 1984, METHOD MICROBIOL, V15, P345

[5]

*BIOM VIT INC, 1992, RAP NFT ID COD

[6]

*BIOM VIT INC, 1992, 20E AN PROF IND

[7] USE OF THE API RAPID NFT SYSTEM FOR IDENTIFYING NONFERMENTATIVE AND FERMENTATIVE MARINE-BACTERIA [J].

BRESCHEL, TS ;

SINGLETON, FL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (01) :21-26

[8] BIOCHEMICAL AND TOXIGENIC PROPERTIES OF VIBRIO-FURNISSII ISOLATED FROM A EUROPEAN EEL FARM [J].

ESTEVE, C ;

AMARO, C ;

BIOSCA, EG ;

GARAY, E .

AQUACULTURE, 1995, 132 (1-2) :81-90

[9] ACCURACY, REPRODUCIBILITY, AND INTERPRETATION OF FATTY-ACID METHYL-ESTER PROFILES OF MODEL BACTERIAL COMMUNITIES [J].

HAACK, SK ;

GARCHOW, H ;

ODELSON, DA ;

FORNEY, LJ ;

KLUG, MJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (07) :2483-2493

[10] IDENTIFICATION OF AQUATIC BURKHOLDERIA (PSEUDOMONAS) CEPACIA BY HYBRIDIZATION WITH SPECIES-SPECIFIC RIBOSOMAL-RNA GENE PROBES [J].

LEFF, LG ;

KERNAN, RM ;

MCARTHUR, JV ;

SHIMKETS, LJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (04) :1634-1636

← 1 2 →