A marked upregulation of uncoupling protein 2 gene expression in adipose tissue of hyperthyroid subjects

被引:16
作者
Hoffstedt, J
Folkesson, R
Wahrenberg, H
Wennlund, A
van Harmelen, V
Arner, P
机构
[1] Huddinge Univ Hosp, Karolinska Inst, CME, Dept Med, S-14186 Huddinge, Sweden
[2] Huddinge Univ Hosp, Karolinska Inst, Dept Geriatr Med, S-14186 Huddinge, Sweden
[3] Huddinge Univ Hosp, Karolinska Inst, Neurotec, S-14186 Huddinge, Sweden
关键词
fat cell; mRNA; polymerase chain reaction; thermogenesis; thyrotoxicosis;
D O I
10.1055/s-2007-978673
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Recently, a family of uncoupling protein (UCP) genes has been discovered. The role of these genes is unknown, but it has been suggested that they are involved in regulating resting metabolic rate. In this study, we hypothesised that thyroid hormone status may influence the expression of UCP2 mRNA. The adipose tissue levels of UCP2 mRNA were measured in eight female subjects before and after treatment for thyrotoxicosis. All subjects in the hyperthyroid condition had markedly enhanced plasma levels of thyroxine (62.0 +/- 6.9 vs. 17.9 +/- 1.7, p = 0.012) and triiodothyronine (37.9 +/- 6.9 vs. 5.9 +/- 0.9, p = 0.012), accelerated heart rate (94 +/- 7 vs. 69 +/- 5, p = 0.012), decreased BMI (24.5 +/- 1.9 vs. 25.1 +/- 1.9, p = 0.025) and decreased percentage body fat (32.8 +/- 4.4 vs. 37.1 +/- 4.5, p = 0.018), as compared to the euthyroid state. Using RT-competitive-PCR, the UCP2 mRNA levels were found to be 2.5-fold upregulated in hyperthyroidism (10.4 +/- 1.7 vs. 4.2 +/- 1.3 amol/mu gRNA, p = 0.012). In contrast, no difference in expression levels of the reference gene 18SrRNA was seen in the hyperthyroid versus the euthyroid state (317 +/- 49 vs. 279 +/- 25 amol/mu gRNA, p = 0.48) but the difference in UCP2 mRNA levels between the hyper- and euthyroid state remained when UCP2 was related to 18SrRNA (p = 0.012). In conclusion, thyrotoxicosis markedly increases the expression of UCP2 mRNA in adipose tissue, which suggests a role for thyroid hormones in the regulation of this uncoupling protein in man.
引用
收藏
页码:475 / 479
页数:5
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