Neuropeptidomic analysis of the brain and thoracic ganglion from the Jonah crab, Cancer borealis

被引：122

作者：

Huybrechts, J

Nusbaum, MP

Vanden Bosch, L

Baggerman, G

De Loof, A

Schoofs, L

机构：

[1] Katholieke Univ Leuven, Lab Dev Physiol & Mol Biol, BE-3000 Louvain, Belgium

[2] Univ Penn, Sch Med, Dept Neurosci, Philadelphia, PA 19104 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 308卷 / 03期

关键词：

Q-TOF; MALDI-TOF imaging; mass spectrometry; peptidomics; Cancer borealis; orcokinin; FMRFamide-related peptides; allatostatins;

D O I：

10.1016/S0006-291X(03)01426-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mass spectrometric methods were applied to determine the peptidome of the brain and thoracic ganglion of the Jonah crab (Cancer borealis). Fractions obtained by high performance liquid chromatography were characterized using MALDI-TOF MS and ESI-Q-TOF MS/MS. In total, 28 peptides were identified within the molecular mass range 750-3000 Da. Comparison of the molecular masses obtained with MALDI-TOF MS with the calculated molecular masses of known crustacean peptides revealed the presence of at least nine allatostatins, three orcokinin precursor derived peptides, namely FDAFTTGFGHS, [Ala(13)]-orcokinin, and [Val(13)]-orcokinin, and two kinins, a tachykinin-related peptide and four FMRFamide-related peptides. Eight other peptides were de novo sequenced by collision induced dissociation on the Q-TOF system and yielded AYNRSFLRFamide, PELDHVFLRFamide or EPLDHVFLRFamide, APQRNFLRFamide, LNPFLRFamide, DVRTPALRLRFamide, and LRNLRFamide, which belong to the FMRFamide related peptide family, as well as NFDEIDRSGFA and NFDEIDRSSFGFV, which display high sequence similarity to peptide sequences within the orcokinin precursor of Orconectes limosus. Our paper is the first (neuro)peptidomic analysis of the crustacean nervous system. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：535 / 544

页数：10

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