Multiple forms of ADP-glucose pyrophosphorylase from tomato leaf

被引：7

作者：

Chen, BY ^{[1
]}

Janes, HW ^{[1
]}

机构：

[1] Rutgers State Univ, Dept Plant Sci, New Brunswick, NJ 08901 USA

来源：

PHYSIOLOGIA PLANTARUM | 1998年 / 103卷 / 04期

关键词：

ADP-glucose pyrophosphorylase; enzyme purification; Lycopersicon esculentum; starch synthesis; tomato;

D O I：

10.1034/j.1399-3054.1998.1030407.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

ADP-glucose pyrophosphorylase (AGP, EC 2.7.7.27) was partially purified from tomato (Lycopeisicon esculentum Mill. cv. Laura) leaf by a procedure previously used for purification of AGP from tomato pericarp. SDS-PAGE and western blot analysis of the final preparation indicated that the leaf enzyme is composed of two subunits of 50 and 54 kDa. Two-dimensional PAGE and western blot analysis of the same preparation, however, revealed at least six isoforms of the large subunit and two isoforms of the small subunit. The leaf AGP is very sensitive to regulation by 3-phosphoglycerate and inorganic phosphate. Its properties are compared to those of AGP from tomato fruit.

引用

页码：491 / 496

页数：6

共 35 条

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