Cellulose-acetate disks as novel substrate for the resynthesis and culture of lichens

被引:8
作者
Backor, M [1 ]
Fahselt, D
机构
[1] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada
[2] Safarik Univ, Dept Bot, Inst Biol & Ecol, Kosice 04167, Slovakia
关键词
D O I
10.1639/09
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cellulose-acetate disks were found convenient for the cultivation of lichen mycobionts and artificial resynthesis and culture of the lichen Cladonia cristatella. These cost-effective disks solve many resynthesis problems, as they are easily sterilized to facilitate mycobiont establishment. They also permit transfer of mycobiont cultures to nutrient-poor agar stimulating hyphal branching which is conducive to lichenization, with minimal carry over of the original medium. Mycobionts on cellulose-acetate disks can be directly inoculated with photobionts and preweighing permits growth of re-synthesized lichens to be monitored easily.
引用
收藏
页码:439 / 442
页数:4
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