Application of preparative high-speed counter-current chromatography/electrospray ionization mass spectrometry for a fast screening and fractionation of polyphenols

被引：51

作者：

Gutzeit, Derek

Winterhalter, Peter

Jerz, Gerold

机构：

[1] Cent Inst Bundeswehr Med Serv, D-85748 Garching, Germany

[2] Tech Univ Carolo Wilhelmina Braunschweig, Inst Food Chem, D-38106 Braunschweig, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1172卷 / 01期

关键词：

preparative HSCCC/ESI-MS-MS; counter-current chromatography; flavonol glycosides; protocatechuic acid; sea buckthorn juice concentrate; Hippophae rhamnoides L. ssp rhamnoides;

D O I：

10.1016/j.chroma.2007.09.066

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study presents the novel direct coupling of preparative high-speed counter-current chromatography with electrospray ionization mass spectrometry (HSCCC/ESI-MS-MS) for continuous MS data acquisition, and 'target'-guided fractionation of polyphenolic compounds from sea buckthorn juice concentrate (Hippophae rhamnoides L. ssp. rhamnoides, Elaeagnaceae). It was demonstrated that a combination of HSCCC and ESI-MS-MS is a versatile tool for fast screening and sensitive detection of bioactive natural products in crude extracts. Sample fractionation will be simplified by knowing the continuous MS data of the complete HSCCC separation. Already during the separation, immediate structural information of the separated compounds is provided. Peak purity of HSCCC is assessable by complete ion traces and fractions can directly be evaporated for further one/two-dimensional NMR analysis. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：40 / 46

页数：7

共 12 条

[1] Semi-industrial isolation of salicin and amygdalin from plant extracts using slow rotary counter-current chromatography [J].