In vitro antiplatelet activity of flavonoids from Leuzea carthamoides

被引:36
作者
Koleckar, Vit [2 ,3 ]
Brojerova, Eliska [3 ]
Rehakova, Zuzana [3 ]
Kubikova, Katerina [3 ]
Cervenka, Frantisek [3 ]
Kuca, Kamil [1 ,2 ,4 ]
Jun, Daniel [1 ,2 ]
Hronek, Miloslav [5 ]
Opletalova, Veronika [6 ]
Opletal, Lubomir [3 ]
机构
[1] Univ Def, Fac Mil Hlth Sci, Dept Toxicol, Hradec Kralove 50001, Czech Republic
[2] Univ Def, Fac Mil Hlth Sci, Ctr Adv Studies, Hradec Kralove 50001, Czech Republic
[3] Charles Univ Prague, Fac Pharm, Dept Pharmaceut Bot & Ecol, CS-50165 Hradec Kralove, Czech Republic
[4] J E Purkinjie Univ, Fac Sci, Dept Chem, Usti Nad Labem, Czech Republic
[5] Charles Univ Prague, Fac Pharm, Dept Biol & Med Sci, CS-50165 Hradec Kralove, Czech Republic
[6] Charles Univ Prague, Fac Pharm, Dept Pharmaceut Chem & Drug Control, CS-50165 Hradec Kralove, Czech Republic
关键词
antiplatelet activity; flavonoids; Leuzea carthamoides; aggregation; plants; quantity;
D O I
10.1080/01480540701688444
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Plants and their secondary metabolites, including flavonoids, exhibit a wide range of biological effects. Consequently, natural substances are receiving an increased attention in medicinal research. Owing to these facts, in vitro antiplatelet activity of ethanol summary extract and four flavonoids from Leuzea carthamoides was determined in human platelet-rich plasma. Arachidonic acid (AA), adenosine diphosphate (ADP), collagen (COL), and thrombin were used as agonists of platelet aggregation. The summary extract showed a significant inhibition of the aggregation induced by COL and ADP. Of the tested flavonoids, eriodictyol (1) and patuletin (2) influenced COL- and AA-induced aggregation. Their IC50 values are presented. Flavonoid glycosides eriodictyol-7-beta-glucopyranoside (3) and 6-hydroxykaempferol-7-O-(6 ''-O-acetyl-beta-D[small cap]-glucopyranoside) (4) were found to be weak antiplatelet agents. These results confirmed the fact that glucosylation decreases the antiplatelet activity. Quantitative composition of tested flavonoids in L. carthamoides extract was also determined. Though two of the tested flavonoids inhibited platelet aggregation, further evaluation of L. carthamoides, in order to discover other antiplatelet active compounds and possible adverse health effects, is needed.
引用
收藏
页码:27 / 35
页数:9
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