Desulfitobacterium sp strain PCE1, an anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene or ortho-chlorinated phenols

被引:207
作者
Gerritse, J [1 ]
Renard, V [1 ]
Gomes, TMP [1 ]
Lawson, PA [1 ]
Collins, MD [1 ]
Gottschal, JC [1 ]
机构
[1] BBSRC,FOOD RES INST,READING LAB,READING RG6 6BZ,BERKS,ENGLAND
关键词
Desulfitobacterium; chlorinated ethenes; chlorinated phenols; reductive dechlorination;
D O I
10.1007/s002030050308
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A strictly anaerobic bacterium, strain PCE1, was isolated from a tetrachloroethene-dechlorinating enrichment culture. Cells of the bacterium were motile curved rods, with approximately four lateral flagella. They possessed a gram-positive type of cell wall and contained cytochrome c. Optimum growth occurred at pH 7.2-7.8 and 34-38 degrees C. The organism grew with L-lactate, pyruvate, butyrate, formate, succinate, or ethanol as electron donors, using either tetrachloroethene, 2-chlorophenol, 2,4,6-trichlorophenol, 3-chloro-4-hydroxy-phenylacetate, sulfite, thiosulfate, or fumarate as electron accepters. Strain PCE1 also grew fermentatively with pyruvate as the sole substrate. L-Lactate and pyruvate were oxidized to acetate. Tetrachloroethene was reductively dechlorinated to trichloroethene and small amounts (< 5%) of cis-1,2-dichloroethene and trans-1,2-dichloroethene. Chlorinated phenolic compounds were dechlorinated specifically at the ol-tho-position. On the basis of 16S rRNA sequence analysis, the organism was identified as a species within the genus Desulfitobacterium, which until now only contained the chlorophenol-dechlorinating bacterium, Desulfitobacterium dehalogenans.
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页码:132 / 140
页数:9
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