Hydroxyapatite reinforced inherent RGD containing silk fibroin composite scaffolds: Promising platform for bone tissue engineering

被引:58
作者
Behera, Sibaram [1 ]
Naskar, Deboki [1 ]
Sapru, Sunaina [1 ]
Bhattacharjee, Promita [2 ]
Dey, Tuli [1 ,3 ]
Ghosh, Ananta K. [1 ]
Mandal, Mahitosh [4 ]
Kundu, Subhas C. [1 ]
机构
[1] Indian Inst Technol, Dept Biotechnol, Kharagpur, W Bengal, India
[2] Indian Inst Technol, Dept Mat Sci, Kharagpur, W Bengal, India
[3] Savitribai Phule Pune Univ, Inst Bioinformat & Biotechnol, Pune, Maharashtra, India
[4] Indian Inst Technol, Sch Med Sci & Technol, Kharagpur, W Bengal, India
关键词
Non-mulberry silk fibroin; HAp; Nanoparticles; Bone tissue engineering; MESENCHYMAL STEM-CELLS; GROWTH-FACTOR DELIVERY; NECROSIS-FACTOR-ALPHA; PROTEIN FIBROIN; IN-VITRO; OSTEOGENIC DIFFERENTIATION; BIOMATERIALS; INTERLEUKIN-1-BETA; OSTEOCONDUCTIVITY; NUCLEATION;
D O I
10.1016/j.nano.2017.02.016
中图分类号
TB3 [工程材料学];
学科分类号
082905 [生物质能源与材料];
摘要
Replacement and repair of ectopic bone defects and traumatized bone tissues are done using porous scaffolds and composites. The prerequisites for such scaffolds include high mechanical strength, osseoconductivity and cytocompatibility. The present work is designed to address such requirements by fabricating a reinforced cytocompatible scaffold. Biocompatible silk protein fibroin collected from tropical non-mulberry tasar silkworm (Antheraea mylitta) is used to fabricate fibroin-hydroxyapatite (HAp) nanocomposite particles using chemical precipitation method. In situ reinforcement of fibroin-HAp nanocomposite and external deposition of HAp particles on fibroin scaffold is carried out for comparative evaluations of bio-physical and biochemical characteristics. HAp deposited fibroin scaffolds provide greater mechanical strength and cytocompatibility, when compared with fibroin-HAp nanoparticles reinforced fibroin scaffolds. Minimal immune responses of both types of composite scaffolds are observed using osteoblast-macrophage co-culture model. Nanocomposite reinforced fibroin scaffold can be tailored further to accommodate different requirements depending on bone type or bone regeneration period. (c) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码:1745 / 1759
页数:15
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