Lymphatic system as a path underlying the spread of lung and gut injury after intestinal ischemia/reperfusion in rats

被引:80
作者
Cavriani, G
Domingos, HV
Soares, AL
Trezena, AG
Ligeiro-Oliveira, AP
Oliveira, RM
Sudo-Hayashi, LS
de Lima, WT
机构
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, Brazil
[2] Inst Butantan, Lab Anaerob Vaccines, Sao Paulo, Brazil
来源
SHOCK | 2005年 / 23卷 / 04期
关键词
gut trauma; lymph; tumor necrosis factor; neutrophils; vascular permeability; lung injury; ARDS; myeloperoxidase; pentoxifylline;
D O I
10.1097/01.shk.0000157303.76749.9b
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
We investigated in rats the influence of the lymphatic system and of tumor necrosis factor (TNF) on the lung inflammation resulting from intestinal ischemia/reperfusion (I/R) performed by 45-min occlusion of the superior mesenteric artery followed by 2 h of reperfusion. A group of rats had the thoracic lymph duct ligated before I/R. In lungs, intestinal I/R evoked a significant neutrophil recruitment, and enhanced microvascular permeability, in addition to generation of TNF in serum. In the gut, there was lowered lactate dehydrogenase (LDH) activity and increased microvascular permeability. Upon lymph duct ligation, I/R rats had a significant reduction of pulmonary neutrophil recruitment and plasma extravasation, in addition to high amounts of TNF in the lymph, contrasting with undetectable levels in the serum. In addition, LDH gut levels in these animals were close to basal values; there was also some (yet significant) reduction of microvascular permeability, suggesting that the ligation of the lymphatic duct exerted some degree of protection against the intestinal injury caused by I/R. In I/R rats, the treatment with pentoxifylline (PTX) reduced TNF in serum and blunted other lung alterations. The gut alterations caused by intestinal I/R were largely blocked by PTX. On the other hand, in I/R rats with lymph duct ligation, PTX exacerbated the reduction of pulmonary neutrophil recruitment, but did not affect pulmonary and intestinal microvascular permeabilities. Similarly, intestinal LDH activity and serum TNF levels were unaffected. Overall, our data show that the pulmonary and gut injuries induced by intestinal I/R are partially dependent on TNF, which is conceivably generated in the injured gut tissue due to intestinal I/R and carried by the lymphatic system. Thus, the mesenteric lymphatic drainage seems to play a role as a path modulator of the pulmonary and intestinal dysfunctions that follow a gut trauma.
引用
收藏
页码:330 / 336
页数:7
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