Improved laboratory confirmation of heparin-induced thrombocytopenia type II - Time course of antibodies and combination of antigen and biologic assays

被引:8
作者
Harenberg, J
Wang, LC
Hoffmann, U
Huhle, G
Feuring, M
机构
[1] Univ Hosp, Dept Med 1, D-68167 Mannheim, Germany
[2] Heidelberg Univ, Fac Clin Med Mannheim, Dept Clin Pharmacol, Heidelberg, Germany
关键词
heparin-induced thrombocytopenia; carbon 14 serotonin release assay; fluorescence-linked immunofiltration assay; heparin; thrombosis;
D O I
10.1309/ET2W-5J8E-MFVU-QYAH
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
We studied whether laboratory confirmation of heparin-induced thrombocytopenia (HIT) can be improved after antigen clearance by determining free antibody and combining the results of an antigenic and a biologic assay. Blood samples taken over 40 days in 14 patients with HIT with thromboembolism underwent fluorescence-linked immunofiltration and the carbon 14-serotonin release assays. Of the 14 patients, 11 showed positive results in both assays at day 1 after stopping heparin. The 3 patients with negative results seroconverted in one or both assays during the subsequent 7 days. Combining the positive results of the assays increased the sensitivity from 85% at day 1 to 100% at day 7. Assay results became negative in all patients within 40 days. The platelet count normalized between days 2 and 9 after withdrawal of heparin. It is assumed that the free antibody can be detected after withdrawal of heparin and after clearance of the platelet factor 4-heparin complex in patients with HIT.
引用
收藏
页码:432 / 438
页数:7
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