A systematic screen for protein-lipid interactions in Saccharomyces cerevisiae

被引：129

作者：

Gallego, Oriol ^{[1
]}

Betts, Matthew J. ^{[1
]}

Gvozdenovic-Jeremic, Jelena ^{[1
]}

Maeda, Kenji ^{[1
]}

Matetzki, Christian ^{[1
]}

Aguilar-Gurrieri, Carmen ^{[1
]}

Beltran-Alvarez, Pedro ^{[1
]}

Bonn, Stefan ^{[1
]}

Fernandez-Tornero, Carlos ^{[1
]}

Jensen, Lars Juhl ^{[1
]}

Kuhn, Michael ^{[1
]}

Trott, Jamie ^{[1
]}

Rybin, Vladimir ^{[2
]}

Mueller, Christoph W. ^{[1
]}

Bork, Peer ^{[1
]}

Kaksonen, Marko ^{[3
]}

Russell, Robert B. ^{[1
]}

Gavin, Anne-Claude ^{[1
]}

机构：

[1] European Mol Biol Lab, Struct & Computat Biol Unit, D-69117 Heidelberg, Germany

[2] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany

[3] European Mol Biol Lab, Prot Express & Purificat Core Facil, Heidelberg, Germany

来源：

MOLECULAR SYSTEMS BIOLOGY | 2010年 / 6卷

关键词：

interactome; lipid-array; network; pleckstrin homology domains; sphingolipids; PLECKSTRIN-HOMOLOGY-DOMAIN; GLOBAL ANALYSIS; PH DOMAIN; BINDING; YEAST; PHOSPHOINOSITIDES; IDENTIFICATION; KINASES; GENOMES; SLM1;

D O I：

10.1038/msb.2010.87

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein-metabolite networks are central to biological systems, but are incompletely understood. Here, we report a screen to catalog protein-lipid interactions in yeast. We used arrays of 56 metabolites to measure lipid-binding fingerprints of 172 proteins, including 91 with predicted lipid-binding domains. We identified 530 protein-lipid associations, the majority of which are novel. To show the data set's biological value, we studied further several novel interactions with sphingolipids, a class of conserved bioactive lipids with an elusive mode of action. Integration of live-cell imaging suggests new cellular targets for these molecules, including several with pleckstrin homology (PH) domains. Validated interactions with Slm1, a regulator of actin polarization, show that PH domains can have unexpected lipid-binding specificities and can act as coincidence sensors for both phosphatidylinositol phosphates and phosphorylated sphingolipids. Molecular Systems Biology 6: 430; published online 30 November 2010; doi:10.1038/msb.2010.87

引用

页数：15

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