The gene encoding the NdhH subunit of type 1 NAD(P)H dehydrogenase is essential to survival of Synechocystis PCC6803

被引：21

作者：

Pieulle, L

Guedeney, G

Cassier-Chauvat, C

Jeanjean, R

Chauvat, F

Peltier, G

机构：

[1] CEA Cadarache, DSV, DEVM, Lab Ecophysiol Photosynth, F-13108 St Paul Les Durance, France

[2] CEA Saclay, Serv Biochim & Genet Mol, F-91191 Gif Sur Yvette, France

[3] CNRS, URA 2096, DBCM DSV, Gif Sur Yvette, France

[4] CNRS, Inst Biol Struct & Microbiol, Chim Bacterienne Lab, F-13402 Marseille 20, France

来源：

FEBS LETTERS | 2000年 / 487卷 / 02期

关键词：

cyanobacteria; Synechocystis PCC6803; NAD(P)H dehydrogenase; ndh gene; temperature-controlled expression;

D O I：

10.1016/S0014-5793(00)02369-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The physiological function of the type 1 NAD(P)H dehydrogenase (Ndh-1) of Synechocystis sp, PCC6803 has been investigated by inactivating the gene ndhH encoding a subunit of the complex, Molecular analysis of independent transformants revealed that all clones were heteroploid, containing both,wild-type and mutant ndhH copies, whatever the metabolic conditions used during genome segregation, including high CO2 concentration. By replacing the chromosomal copy of the ndhH gene by a plasmidial copy under the control of a temperature-controlled promoter, we induce a conditional phenotype, growth being only possible at high temperature. This clearly shows for the first time that an ndh gene is indispensable to the survival of Synechocystis sp, PCC6803. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：272 / 276

页数：5

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