Functional interaction between RNA polymerase α subunit C-terminal domain and σ70 in UP-element- and activator-dependent transcription

被引：64

作者：

Chen, H

Tang, H

Ebright, RH ^{[1
]}

机构：

[1] Rutgers State Univ, Howard Hughes Med Inst, Piscataway, NJ 08854 USA

[2] Rutgers State Univ, Waksman Inst, Piscataway, NJ 08854 USA

[3] Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA

来源：

MOLECULAR CELL | 2003年 / 11卷 / 06期

关键词：

D O I：

10.1016/S1097-2765(03)00201-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that the Escherichia coli RNA polymerase (RNAP) subunit C-terminal domain (alphaCTD) functionally interacts with sigma(70) at a subset of UP-element- and activator-dependent promoters, we define the determinants of aCTD and sigma(70) required for the interaction, and we present a structural model for the interaction. The alphaCTD-sigma(70) interaction spans the upstream promoter and core promoter, thereby linking recognition of UP-elements and activators in the upstream promoter with recognition of the -35 element in the core promoter. We propose that the alphaCTD-sigma(70) interaction permits UP-elements and activators not only to "recruit" RNAP through direct interaction with aCTD, but also to "remodel" RNAP-core-promoter interaction through indirect, alphaCTD-bridged interactions with sigma(70).

引用

页码：1621 / 1633

页数：13

共 47 条

[1] Upstream A-tracts increase bacterial promoter activity through interactions with the RNA polymerase α subunit [J].