Fusarium solani pisi cutinase

被引：130

作者：

Egmond, MR ^{[1
]}

de Vlieg, J ^{[1
]}

机构：

[1] Unilever Res Labs, NL-3133 AT Vlaardingen, Netherlands

来源：

BIOCHIMIE | 2000年 / 82卷 / 11期

关键词：

Fusarium solani pisi; cutinase; crystal structure; kinetic behaviour;

D O I：

10.1016/S0300-9084(00)01183-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cutinase from Fusarium solani pisi has been studied extensively with respect to its structural and functional properties. The crystal structure of the enzyme was solved to high atomic resolution (1 angstrom), while data on structural dynamics have been obtained from detailed NMR studies. Functional data were mainly derived from kinetic studies using substrate analogues that simplify the kinetic behaviour. The properties of wild-type cutinase are reviewed and discussed in relation with the effects brought about by site-directed variants of the enzyme. (C) 2000 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier SAS.

引用

页码：1015 / 1021

页数：7

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