Targeted mapping approaches to identify DNA markers linked to the Rfp1 restorer gene for the 'Polima' CMS of canola (Brassica napus L.)

被引:17
作者
Jean, M
Brown, GG
Landry, BS
机构
[1] DNA Landmarks, St Jean, PQ J3B 6Z1, Canada
[2] McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada
关键词
targeted mapping; RFLP; RAPD; Brassica napus; Polima CMS; nearly isogenic line; bulked segregant analysis;
D O I
10.1007/s001220050913
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
We have used two targeting approaches [pairs of nearly isogenic lines (NILs) and bulked segregant analysis] to identify DNA markers linked to the Rfp1 restorer gene for the pol CMS of canola (Brassica napus L.). We were able to target the Rfp1 locus as efficiently by comparing NILs as by bulked segregant analysis, and it was demonstrated in this instance that double-screening strategies could significantly improve the overall targeting efficiency. The chance occurrence of shared homozygosity at specific unlinked chromosomal regions in the bulks was found to limit the efficiency of bulked segregant analysis, while the efficiency of NIL comparison was limited by residual DNA from the donor cultivar at scattered sites throughout the genome of the NILs.
引用
收藏
页码:431 / 438
页数:8
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