Efficient FLP recombination in mouse ES cells and oocytes

被引：115

作者：

Schaft, J

Ashery-Padan, R

van der Hoeven, F

Gruss, P

Stewart, AF

机构：

[1] EMBL, Gene Express Program, D-69117 Heidelberg, Germany

[2] Max Planck Inst Biophys Chem, Dept Mol Cell Biol, D-37077 Gottingen, Germany

来源：

GENESIS | 2001年 / 31卷 / 01期

关键词：

Cre; site specific recombination; genetic engineering; transgenesis; homologous recombination;

D O I：

10.1002/gene.1076

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLIP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLIP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice. (C) 2001 Wiley-Liss, Inc.

引用

页码：6 / 10

页数：5

共 28 条

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