Hypoosmolarity and glutamine increased the beta-actin gene transcription in isolated rat hepatocytes

被引：24

作者：

Husson, A ^{[1
]}

Quillard, M ^{[1
]}

Fairand, A ^{[1
]}

Chedeville, A ^{[1
]}

Lavoinne, A ^{[1
]}

机构：

[1] UFR MED PHARM ROUEN, IFRMP, GBPDN, F-76803 ST ETIENNE, FRANCE

来源：

FEBS LETTERS | 1996年 / 394卷 / 03期

关键词：

hydration state; glutamine; hepatocyte; transcription; beta-actin;

D O I：

10.1016/0014-5793(96)00985-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanism of action of hydration state was studied on beta-actin gene expression in isolated hepatocytes, Results obtained with Northern blot analysis and run on transcription assays show that hypoosmolarity increased and hyperosmolarity decreased the beta-actin mRNA level through a corresponding modulation of the rate of the gene transcription, Glutamine, which is known to induce cell swelling, also increased the beta-actin mRNA level in a dose-dependent manner and induced a stimulation of the beta-actin gene transcription, Thus, cell hydration state regulates gene expression in the liver through a transcriptional mechanism.

引用

页码：353 / 355

页数：3

共 12 条

[1] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[2] NUMBER AND EVOLUTIONARY CONSERVATION OF ALPHA-TUBULIN AND BETA-TUBULIN AND CYTOPLASMIC BETA-ACTIN AND GAMMA-ACTIN GENES USING SPECIFIC CLONED CDNA PROBES [J].

CLEVELAND, DW ;

LOPATA, MA ;

MACDONALD, RJ ;

COWAN, NJ ;

RUTTER, WJ ;

KIRSCHNER, MW .

CELL, 1980, 20 (01) :95-105

[3] INCREASE OF C-JUN MESSENGER-RNA UPON HYPOOSMOTIC CELL SWELLING OF RAT HEPATOMA-CELLS [J].

FINKENZELLER, G ;

NEWSOME, W ;

LANG, F ;

HAUSSINGER, D .

FEBS LETTERS, 1994, 340 (03) :163-166

[4] VARIOUS RAT ADULT TISSUES EXPRESS ONLY ONE MAJOR MESSENGER-RNA SPECIES FROM THE GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE MULTIGENIC FAMILY [J].

FORT, P ;

MARTY, L ;

PIECHACZYK, M ;

ELSABROUTY, S ;

DANI, C ;

JEANTEUR, P ;

BLANCHARD, JM .

NUCLEIC ACIDS RESEARCH, 1985, 13 (05) :1431-1442

[5] THE 18S RIBOSOMAL-RNA SEQUENCE OF THE SEA-ANEMONE ANEMONIA-SULCATA AND ITS EVOLUTIONARY POSITION AMONG OTHER EUKARYOTES [J].

HENDRIKS, L ;

VANDEPEER, Y ;

VANHERCK, M ;

NEEFS, JM ;

DEWACHTER, R .

FEBS LETTERS, 1990, 269 (02) :445-449

[6]

MARCHAND JC, 1979, BIOCHIMIE, V61, P1273

[7] MODULATION OF PHOSPHOENOLPYRUVATE CARBOXYKINASE MESSENGER-RNA LEVELS BY THE HEPATOCELLULAR HYDRATION STATE [J].

NEWSOME, WP ;

WARSKULAT, U ;

NOE, B ;

WETTSTEIN, M ;

STOLL, B ;

GEROK, W ;

HAUSSINGER, D .

BIOCHEMICAL JOURNAL, 1994, 304 :555-560

[8] Glutamine increases argininosuccinate synthetase mRNA levels in rat hepatocytes - The involvement of cell swelling [J].

Quillard, M ;

Husson, A ;

Lavoinne, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 236 (01) :56-59

[9] CHANGES IN LEVELS OF ARGININOSUCCINATE LYASE MESSENGER-RNA DURING INDUCTION BY GLUCAGON AND CYCLIC-AMP IN CULTURED FETAL-RAT HEPATOCYTES [J].

RENOUF, S ;

BUQUET, C ;

FAIRAND, A ;

BENAMAR, M ;

HUSSON, A .

BIOCHEMICAL JOURNAL, 1993, 291 :609-613

[10] HEPATOCYTE SWELLING LEADS TO RAPID DECREASE OF THE G-ACTIN TOTAL ACTIN RATIO AND INCREASES ACTIN MESSENGER-RNA LEVELS [J].

THEODOROPOULOS, PA ;

STOURNARAS, C ;

STOLL, B ;

MARKOGIANNAKIS, E ;

LANG, F ;

GRAVANIS, A ;

HAUSSINGER, D .

FEBS LETTERS, 1992, 311 (03) :241-245

← 1 2 →