Microsatellite loci for paternity analysis in the fathead minnow, Pimephales promelas (Teleostei: Cyprinidae)

被引：31

作者：

Bessert, ML ^{[1
]}

Ortí, G ^{[1
]}

机构：

[1] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA

来源：

MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 04期

关键词：

Cyprinidae; enriched library; microsatellite; paternity analysis; Pimephales promelas; primers;

D O I：

10.1046/j.1471-8286.2003.00501.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Many recent studies have employed molecular markers to uncover important aspects of mating systems in teleost fishes. The fathead minnow (Pimephales promelas) is a nest-building North American cyprinid that spawns multiply and exhibits exclusive male parental care. A battery of microsatellite markers was developed to analyse paternity in this species. The seven characterized loci possess four to 31 alleles and expected heterozygosities of 0.455-0.974. In combination, they elicit an exclusion probability of 0.999, a desirable level for paternity analysis. In addition, cross-amplifications were conducted to test primer efficacy in 13 other taxa, including two congeners.

引用

页码：532 / 534

页数：3

共 5 条

[1]

Genetic perspectives on the natural history of fish mating systems [J].