Isolation and characterization of microsatellite markers in Bonelli's eagle (Hieraaetus fasciatus)

被引:9
作者
Mira, S
Wolff, K
Cancela, ML [1 ]
机构
[1] Univ Algarve, CCMar, P-8005139 Faro, Portugal
[2] Univ Newcastle Upon Tyne, Sch Biol, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
来源
MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 03期
关键词
Bonelli's eagle; enriched libraries; microsatellite markers;
D O I
10.1111/j.1471-8286.2005.00967.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To obtain polymorphic molecular markers for population genetics and conservation studies in Bonelli's eagle populations, we screened a partial genomic library enriched for microsatellites with di- and tri-nucleotide motifs [(GT) (CT) (AAC) and (GCC)]. A total of 15 polymorphic markers were obtained. The number of alleles ranged from two to eight. These markers will be very useful for paternity tests and population structure studies as well as for evaluating the outcome of conservation programs.
引用
收藏
页码:493 / 495
页数:3
相关论文
共 8 条
[1]  
BELKHIR K, GENETIC LOGICAL WIND
[2]  
Billotte N., 1999, Fruits (Paris), V54, P277
[3]  
Edwards KJ, 1996, BIOTECHNIQUES, V20, P758
[4]  
Kijas J. M. H., 1994, Biotechniques, V16, P657
[5]   Subtraction with 3′ modified oligonucleotides eliminates amplification artifacts in DNA libraries enriched for microsatellites [J].
Koblizkova, A ;
Dolezel, J ;
Macas, J .
BIOTECHNIQUES, 1998, 25 (01) :32-+
[6]  
Krawetz S, 2000, BIOINFORMATICS METHO
[7]   Eighteen polymorphic microsatellite markers for the highly endangered Spanish imperial eagle (Aquila adalberti) and related species [J].
Martínez-Cruz, B ;
David, VA ;
Godoy, JA ;
Negro, JJ ;
O'Brien, SJ ;
Johnson, WE .
MOLECULAR ECOLOGY NOTES, 2002, 2 (03) :323-326
[8]   PRESERVATION OF AVIAN BLOOD AND TISSUE SAMPLES FOR DNA ANALYSES [J].
SEUTIN, G ;
WHITE, BN ;
BOAG, PT .
CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE, 1991, 69 (01) :82-90