Gibberellin biosynthetic pathway in Gibberella fujikuroi:: Evidence for a gene cluster

被引:146
作者
Tudzynski, B [1 ]
Hölter, K [1 ]
机构
[1] Univ Munster, Inst Bot, D-48149 Munster, Germany
关键词
Gibberella fujikuroi; gibberellin biosynthesis; gene cluster; cytochrome P450 monooxygenase genes; geranylgeranyl diphosphate synthase gene; copalyl diphosphate synthase gene; gene expression;
D O I
10.1006/fgbi.1998.1095
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Differential screening of a Gibberella fujikuroi cDNA library was used to successfully clone and identify genes involved in the pathway of gibberellin biosynthesis, Several cDNA clones that hybridized preferentially to a cDNA probe prepared from mycelium induced for gibberellin production were isolated and characterized, The deduced amino acid sequences of two (identical) clones contained the conserved heme-binding motif of cytochrome P450 monooxygenases (FXXGXXXCXG), One of these cDNA fragments was used as a homologous probe for the screening of a genomic library, A hybridizing 6.7-kb genomic Sail fragment was cloned into pUC19, The sequencing of this clone revealed that a second cytochrome P450 monooxygenase gene was closely linked to the first one, Since at least four cytochrome P450 monooxygenase-catalyzed steps are involved in the synthesis of gibberellins, chromosome walking was performed to find a further gene of this family or other genes involved in gibberellin pathway. Next to the two P450 monooxygenase genes, a putative geranylgeranyl diphosphate synthase gene, the copalyl diphosphate synthase gene, which is the first specific gene of the gibberellin pathway, and a third P450 monooxygenase gene were identified, These results suggest that at least some of the genes involved in the biosynthesis of gibberellins are closely linked in a gene cluster in G. fujikuroi, as has been recently found for other "dispensable" pathways in fungi, (C) 1998 Academic Press.
引用
收藏
页码:157 / 170
页数:14
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