DNA microarrays and beyond: completing the journey from tissue to cell

被引：104

作者：

Mills, JC

Roth, KA

Cagan, RL ^{[1
]}

Gordon, JI

机构：

[1] Washington Univ, Sch Med, Dept Mol Biol & Pharmacol, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

来源：

NATURE CELL BIOLOGY | 2001年 / 3卷 / 08期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/35087108

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

For the cell biologist, identifying changes in gene expression using DNA microarrays is,just the start of along journey from tissue to cell. We discuss how chip users can first filter noise (false-positives) from daunting microarray datasets. Combining laser capture microdissection with real-time polymerase chain reaction and reverse transcription is a helpful follow-up step that allows expression of selected genes to be quantified in populations of recovered cells. The voyage from chip to single cell can be completed using sensitive new in situ hybridization and immunohistochemical methods based on tyramide signal amplification.

引用

页码：E175 / E178

页数：4

共 27 条

[1] Banks RE, 1999, ELECTROPHORESIS, V20, P689, DOI 10.1002/(SICI)1522-2683(19990101)20:4/5<689::AID-ELPS689>3.0.CO
[2] 2-J
[3] Gene expression informatics - it's all in your mine
Bassett, DE
Eisen, MB
Boguski, MS
[J]. NATURE GENETICS, 1999, 21 (Suppl 1) : 51 - 55
[4] Quantitative analysis of mRNA amplification by in vitro transcription
Baugh, L. R.
Hill, A. A.
Brown, E. L.
Hunter, Craig P.
[J]. NUCLEIC ACIDS RESEARCH, 2001, 29 (05)
[5] One-stop shop for microarray data - Is a universal, public DNA-microarray database a realistic goal?
Brazma, A
Robinson, A
Cameron, G
Ashburner, M
[J]. NATURE, 2000, 403 (6771) : 699 - 700
[6] Computational methods for the identification of differential and coordinated gene expression
Claverie, JM
[J]. HUMAN MOLECULAR GENETICS, 1999, 8 (10) : 1821 - 1832
[7] Identification of genes differentially regulated by interferon α, β, or γ using oligonucleotide arrays
Der, SD
Zhou, AM
Williams, BRG
Silverman, RH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) : 15623 - 15628
[8] Laser capture microdissection
EmmertBuck, MR
Bonner, RF
Smith, PD
Chuaqui, RF
Zhuang, ZP
Goldstein, SR
Weiss, RA
Liotta, LA
[J]. SCIENCE, 1996, 274 (5289) : 998 - 1001
[9] Goldsworthy SM, 1999, MOL CARCINOGEN, V25, P86, DOI 10.1002/(SICI)1098-2744(199906)25:2<86::AID-MC2>3.3.CO
[10] 2-W

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