Periodontitis decreases the antiatherogenic potency of high density lipoprotein

被引:128
作者
Pussinen, PJ [1 ]
Jauhiainen, M
Vilkuna-Rautiainen, T
Sundvall, J
Vesanen, M
Palosuo, T
Alfthan, G
Asikainen, S
机构
[1] Univ Helsinki, Inst Dent, Helsinki, Finland
[2] Univ Helsinki, Cent Hosp, Dept Oral & Maxillofacial Dis, Helsinki, Finland
[3] Natl Publ Hlth Inst, Dept Mol Med, Helsinki, Finland
[4] Natl Publ Hlth Inst, Dept Hlth & Funct Abil, Helsinki, Finland
[5] Umea Univ, Dept Med & Odontol, Adhes Ctr Oral Microbiol, Umea, Sweden
关键词
HDL metabolism; atherosclerosis; reverse cholesterol transport; cholesterol efflux; inflammation;
D O I
10.1194/jlr.M300250-JLR200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Periodontitis, a consequence of persistent bacterial infection and chronic inflammation, has been suggested to predict coronary heart disease (CHD). The aim of this study was to investigate the impact of periodontitis on HDL structure and antiatherogenic function in cholesterol efflux in vitro. HDL was isolated from 30 patients (age 43.6 +/- 6.1 years, mean +/- SD) with periodontitis before and after (3.2 +/- 1.4 months) periodontal treatment. The capacity of HDL for cholesterol efflux from macrophages (RAW 264.7), HDL composition, and key proteins of HDL metabolism were determined. After periodontal treatment, phospholipid transfer protein (PLTP) activity was 6.2% (P < 0.05) lower, and serum HDL cholesterol concentration, PLTP mass, and cholesteryl ester transfer protein activity were 10.7% (P < 0.001), 7.1% (P = 0.078), and 19.4% (P < 0.001) higher, respectively. The mean HDL2/HDL3 ratio increased from 2.16 +/- 0.87 to 3.56 +/- 0.48 (P < 0.05). HDL total phospholipid mass and sphingomyelin-phosphatidyl-choline ratio were 7.4% (P < 0.05) and 36.8% (P < 0.001) higher, respectively. The HDL-mediated cholesterol efflux tended to be higher after periodontal treatment; interestingly, this increase was significant (P < 0.05) among patients whose C-reactive protein decreased (53.7% reduction, P = 0.015) and who were positive by PCR for Actinobacillus actinomycetemcomitans. These results suggest that periodontitis causes similar, but milder, changes in HDL metabolism than those that occur during the acute-phase response and that periodontitis may diminish the antiatherogenic potency of HDL, thus increasing the risk for CHD.
引用
收藏
页码:139 / 147
页数:9
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